Super Mario Bros.
From SmashWiki, the Super Smash Bros. wiki
[edit] Super Smash Bros. series
Mario and his brother Luigi were the only two characters representing their series in Super Smash Bros., and have become staples in the games ever since. Princess Peach and Bowser were meant to be included in the original Super Smash Bros., but were not added, due to time constraints and limited memory space. However, they are playable in both Super Smash Bros. Melee and Super Smash Bros. Brawl.
In addition, many items from this game, namely the Super Mushroom, the Fire Flower, the Starman, the Springboard, and the Green and Red Shells made their first initial appearances in this game. Other characters and enemies, such as Toad, Goomba, Koopa Troopa, etc. appeared in the Super Smash Bros. series either as trophies, common enemies, or parts of character's attacks.
Also, the Mushroom Kingdom stage in Super Smash Bros. and Mushroom: Kingdom stage in Super Smash Bros. Melee are based graphically on how they looked in Super Mario Bros. In addition, Brawl's Mushroomy Kingdom is based on the look of the game's World 1-1 and World 1-2.
[edit] As a Masterpiece
Super Mario Bros. is one of the selectable Masterpieces in Super Smash Bros. Brawl.
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